Journal: PLoS ONE
Article Title: 9- cis -Retinoic Acid and Troglitazone Impacts Cellular Adhesion, Proliferation, and Integrin Expression in K562 Cells
doi: 10.1371/journal.pone.0093005
Figure Lengend Snippet: Cells were cultured with vehicle ( white bar ), 1 μM 9- cis- RA ( black bar ), 15 μM troglitazone ( gray bar ), or 15 μM and 1 μM troglitazone and 9- cis -RA ( hatched bar ), respectively, for 72 hrs. BrdU incorporation was assessed by ELISA and proliferation values were normalized using Percent Vehicle = (Abs (treatment) /Abs (vehicle) ) X 100. Results are shown as means ± SD, n = 6, LSD was used to test for differences among groups. Means followed by the same letter are not significantly different (P = 0.01).
Article Snippet: The ELISA-based BrdU cell proliferation kit was purchased from Chemicon International (Temecula, CA).
Techniques: Cell Culture, BrdU Incorporation Assay, Enzyme-linked Immunosorbent Assay